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唐国涛,张汉永,黄锦荣,蔡梅玲,朱昔娇,罗万业,邵萍,吴明芳
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梅州市林业科学研究所 514011,梅州市林业科学研究所 514011,梅州市林业科学研究所 514011,梅州市林业科学研究所 514011,梅州市林业科学研究所 514011,梅州市林业科学研究所 514011,梅州市林业科学研究所 514011,梅州市林业科学研究所 514011
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摘要: |
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以油茶（Camellia oleifera）种子胚芽和2 a生扦插苗带侧芽的嫩枝作外植体进行组织培养技术研究。结果表明：适合油茶种子胚芽诱导的培养基为MS+6BA 2.0 mg/L+NAA 0.2 mg/L+GA3 1.0 mg/L+蔗糖30 g/L+卡拉胶6.5 g/L，适宜的增殖培养基为MS+6BA 1.5 mg/L+NAA 0.1 mg/L+GA3 1.0 mg/L+蔗糖30 g/L+卡拉胶6.5 g/L，增殖倍数为3.50；适合油茶不定芽诱导的培养基为MS+6BA 1.5 mg/L+IAA 2.0 mg/L+GA3 1.0 mg/L+蔗糖30 g/L+卡拉胶6.5 g/L，适宜的增殖培养基为MS+6BA 2.0 mg/L+IAA 1.0 mg/L+GA3 1.0 mg/L+蔗糖30 g/L+卡拉胶6.5 g/L，增殖倍数为3.96。木质化程度已较高的油茶组培茎芽适宜的生根基质为细沙，生根率达95%。
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关键词:
油茶；组织培养；繁殖技术
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DOI：
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分类号：
S722.3 7
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基金项目：广东省梅州市科技局农业公关项目“油茶优良无性系组培快繁技术研究” |
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A Preliminary Study on Tissue Culture Technology of Camellia oleifera (Forestry Research Institute of Meizhou city 514011) |
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Tang Guo-tao,,,,,, and
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Abstract: |
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Seed embryo or 2yearold cuttings sprout shooting of Camellia oleifera were used as explants for tissue culture propagation technology research. The results were as follows: the suitable induced medium of C. oleifera seed embryo was MS 6BA 2.0 mg/L NAA 0.2 mg/L GA3 1.0 mg/L sucrose 30 g/L carrageenan 6.5 g/L, and its suitable proliferation medium was MS 6BA 1.5 mg/L NAA 0.1 mg/L GA3 1.0 mg/L sucrose 30 g/L carrageenan 6.5 g/L, the multiplication rate was 3.50. The suitable induced medium of axillary bud was MS 6BA 1.5 mg/L IAA 2.0 mg/L GA3 1.0 mg/L sucrose 30 g/L carrageenan 6.5 g/L, and its suitable proliferation medium was MS 6BA 2.0 mg/L IAA 1.0 mg/L GA3 1.0 mg/L sucrose 30 g/L carrageenan 6.5 g/L, the multiplication rate was 3.96. The fine sand was a suitable matrixes for rooting of highly lignification shoots， the rooting rate was 95%.
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Key words:
Camellia oleifera；tissue culture；breeding technology
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